5OW0
Crystal structure of an electron transfer flavoprotein from Geobacter metallireducens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-08 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97929 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.730, 72.750, 133.881 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.736 - 1.700 |
R-factor | 0.138 |
Rwork | 0.136 |
R-free | 0.16740 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.539 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | AutoSol |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.630 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.092 | 0.584 |
Rpim | 0.038 | 0.250 |
Number of reflections | 59506 | 5846 |
<I/σ(I)> | 15.9 | 3.9 |
Completeness [%] | 99.7 | 99.6 |
Redundancy | 12.8 | 2 |
CC(1/2) | 0.998 | 0.918 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | Mother liquor: 15% (w/v) PEG 3350, 0.1 M HEPES, pH 7.5 mixed equal volumes of 10 mg/ml EtfAB in 300 mM NaCl, 20 mM HEPES, pH 7.5 |