5OVY
Crystal structure of MAB_4384 tetR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.800, 100.820, 56.090 |
| Unit cell angles | 90.00, 105.83, 90.00 |
Refinement procedure
| Resolution | 36.580 - 1.900 |
| R-factor | 0.1865 |
| Rwork | 0.185 |
| R-free | 0.21350 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.452 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.580 | 1.968 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.048 | 0.740 |
| Number of reflections | 31705 | 3193 |
| <I/σ(I)> | 11.09 | 1.06 |
| Completeness [%] | 92.1 | 93.44 |
| Redundancy | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100mM cacodylate pH6.5, 0,2 M MgCl2, 16 pourcent PEG 8000, 5 pourcent dimethylsulfoxide |
