5OVK
Crystal structure MabA bound to NADPH from M. smegmatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-26 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.190, 69.350, 106.940 |
| Unit cell angles | 90.00, 91.85, 90.00 |
Refinement procedure
| Resolution | 39.034 - 1.450 |
| R-factor | 0.1725 |
| Rwork | 0.172 |
| R-free | 0.19690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ovl |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.760 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.034 | 1.502 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmeas | 0.097 | 1.750 |
| Number of reflections | 138568 | 12845 |
| <I/σ(I)> | 14.83 | 1.05 |
| Completeness [%] | 89.1 | 84.6 |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M BIS-TRIS pH 5.5, 0.2 M NaCl and 25% PEG 3350 |
