5OVK
Crystal structure MabA bound to NADPH from M. smegmatis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-26 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.190, 69.350, 106.940 |
Unit cell angles | 90.00, 91.85, 90.00 |
Refinement procedure
Resolution | 39.034 - 1.450 |
R-factor | 0.1725 |
Rwork | 0.172 |
R-free | 0.19690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ovl |
RMSD bond length | 0.005 |
RMSD bond angle | 0.760 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.034 | 1.502 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmeas | 0.097 | 1.750 |
Number of reflections | 138568 | 12845 |
<I/σ(I)> | 14.83 | 1.05 |
Completeness [%] | 89.1 | 84.6 |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M BIS-TRIS pH 5.5, 0.2 M NaCl and 25% PEG 3350 |