5OUQ
Structure of TgPLP1 MACPF domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.072 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 159.490, 185.740, 104.190 |
| Unit cell angles | 90.00, 121.48, 90.00 |
Refinement procedure
| Resolution | 56.350 - 5.110 |
| R-factor | 0.2872 |
| Rwork | 0.287 |
| R-free | 0.29790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5oup |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.638 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2283: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 56.350 |
| High resolution limit [Å] | 5.110 |
| Number of reflections | 10514 |
| <I/σ(I)> | 8.2 |
| Completeness [%] | 99.7 |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M HEPES pH7.5, 10% alcohol mixture, 15% PEG 1000, 15% PEG 3350, 15% MPD |
