5OUG
Humanized alpha-AChBP (acetylcholine binding protein) in complex with lobeline and allosteric binder fragment 4.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-10 |
Detector | DECTRIS PILATUS3 X 1M |
Wavelength(s) | 0.91983 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 68.673, 119.806, 84.543 |
Unit cell angles | 90.00, 107.29, 90.00 |
Refinement procedure
Resolution | 80.720 - 2.570 |
R-factor | 0.2158 |
Rwork | 0.214 |
R-free | 0.24000 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.595 |
Data reduction software | SCALA |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 81.610 | 2.670 |
High resolution limit [Å] | 2.570 | 2.570 |
Rpim | 0.083 | 1.350 |
Number of reflections | 41656 | |
<I/σ(I)> | 11.4 | |
Completeness [%] | 99.8 | |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1 M HEPES pH 7.5, 8% ethylene glycol and 10% PEG8000. |