5OSX

Translation initiation factor 4E in complex with m7G(5'S)ppp(5'S)G mRNA 5' cap analog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918
Spacegroup name	P 1
Unit cell lengths	37.843, 37.855, 145.987
Unit cell angles	86.56, 84.56, 77.05

Refinement procedure

Resolution	48.405 - 1.920
R-factor	0.2259
Rwork	0.224
R-free	0.26890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l8b
RMSD bond length	0.009
RMSD bond angle	0.900
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	1.920	1.920
Rmerge	0.058	0.358
Number of reflections	57473	9143
<I/σ(I)>	10.08	2.04
Completeness [%]	95.2	94
Redundancy	2.43	2.34
CC(1/2)	0.997	0.822

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1M BIS-TRIS propane pH 9.0, 20% v/v PEG MME 550