5OSQ

ZP-N domain of mammalian sperm receptor ZP3 (crystal form II, processed in P21221)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-07
Detector	MARRESEARCH
Wavelength(s)	0.95350
Spacegroup name	P 21 2 21
Unit cell lengths	91.340, 92.160, 140.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.898 - 2.050
R-factor	0.2099
Rwork	0.209
R-free	0.24110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3d4c
RMSD bond length	0.011
RMSD bond angle	0.783
Data reduction software	XDS (Jun 1, 2017 BUILT=20170720)
Data scaling software	XDS (Jun 1, 2017 BUILT=20170720)
Phasing software	PHASER (2.6.0)
Refinement software	PHENIX (1.12-2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.100
High resolution limit [Å]	2.050	2.050
Number of reflections	75254	5501
<I/σ(I)>	13.09	0.67
Completeness [%]	99.9	99.9
Redundancy	7.43	7.51
CC(1/2)	0.999	0.188

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	SAMPLE: 15MG/ML PROTEIN IN 0.05M SODIUM CHLORIDE, 0.01M TRIS-HCL, PH7.2, 0.001M MALTOSE. RESERVOIR: 10% PEG6000, 0.2M CALCIUM CHLORIDE, 0.1M TRIS-HCL, PH7.0. SAMPLE TO RESERVOIR RATIO IN DROP: 1:1, PH7.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293.0K