5OSH
Structure of retromer VPS29-VPS35C subunits complexed with RidL N-terminal domain (1-236)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-09-21 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 90.201, 173.243, 445.775 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 222.890 - 4.300 |
R-factor | 0.2566 |
Rwork | 0.254 |
R-free | 0.31090 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.374 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | autoSHARP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 222.890 | 222.890 | 3.190 |
High resolution limit [Å] | 3.000 | 8.880 | 3.000 |
Rmerge | 0.094 | 0.030 | 2.315 |
Rmeas | 0.101 | 0.032 | 2.517 |
Total number of observations | 651993 | ||
Number of reflections | 93761 | 3671 | 14849 |
<I/σ(I)> | 12.6 | 56.11 | 0.74 |
Completeness [%] | 99.0 | 98.4 | 97.8 |
Redundancy | 6.954 | 6.772 | 6.546 |
CC(1/2) | 0.999 | 0.999 | 0.341 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.1 M sodium chloride 0.1 M Tris pH 8.0 4-8% PEG6000. |