5OQT

Crystal structure of a bacterial cationic amino acid transporter (CAT) homologue

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9686
Spacegroup name	P 21 21 21
Unit cell lengths	76.923, 83.148, 119.744
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.720 - 2.860
R-factor	0.2178
Rwork	0.215
R-free	0.27110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gia
RMSD bond length	0.010
RMSD bond angle	1.100
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.720	2.960
High resolution limit [Å]	2.860	2.860
Rmerge	0.152	1.789
Rpim	0.077	0.842
Number of reflections	18307	1770
<I/σ(I)>	6.3
Completeness [%]	99.0	100
Redundancy	5.2	5.3
CC(1/2)	0.860	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	4	293.15	28-34 % PEG 400, 0.1 M sodium acetate pH 4.0, 0.1 M potassium fluoride, containing 10 mM of the amino acid ligand.