5OQR
Crystal structure of the S. pombe condensin Cnd3-Cnd2 subcomplex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-30 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.977 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 88.407, 142.113, 176.917 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.059 - 2.610 |
R-factor | 0.2238 |
Rwork | 0.223 |
R-free | 0.25760 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.568 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.060 | 2.670 |
High resolution limit [Å] | 2.610 | 2.610 |
Rmerge | 0.211 | 1.482 |
Rpim | 0.060 | 0.425 |
Number of reflections | 68083 | 4542 |
<I/σ(I)> | 11.2 | 1.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.4 | 13 |
CC(1/2) | 0.993 | 0.678 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 280 | 3 to 4% (w/v) PEG 4000, 0.1 M Na citrate pH 5.2 to 5.4, 0.2 M Na acetate |