5OQD
PHD2 and winged-helix domain of Polycomblike
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-04 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.00005 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 307.423, 53.119, 86.839 |
| Unit cell angles | 90.00, 105.47, 90.00 |
Refinement procedure
| Resolution | 83.694 - 2.447 |
| R-factor | 0.2385 |
| Rwork | 0.236 |
| R-free | 0.27800 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.406 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.694 | |
| High resolution limit [Å] | 2.447 | |
| Rpim | 0.030 | 0.371 |
| Number of reflections | 49847 | |
| <I/σ(I)> | 17.09 | |
| Completeness [%] | 98.7 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 12.5 % PEG 3350, 50 mM Potassium Phosphate (dibasic) and 2 mM Manganese Chloride |
