5OQ0
Crystal structure of transthyretin mutant 87-110-117
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-10 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.973186 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 42.249, 67.044, 83.566 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.783 - 1.940 |
| R-factor | 0.2296 |
| Rwork | 0.228 |
| R-free | 0.26710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wo0 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.944 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.290 | 2.010 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.073 | 0.916 |
| Rpim | 0.030 | 0.514 |
| Number of reflections | 9125 | |
| <I/σ(I)> | 13 | 1.6 |
| Completeness [%] | 97.4 | 80.5 |
| Redundancy | 7.2 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M sodium phosphate (pH 7.5), 2.2 M ammonium sulphate |
