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5OPQ

A 3,6-anhydro-D-galactosidase produced by Zobellia galactanivorans. This is an exo-lytic enzyme that hydrolyzes terminal 3,6-anhydro-D-galactose from the non-reducing end of carrageenan oligosaccharides.

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsESRF BEAMLINE ID14-4
Synchrotron siteESRF
BeamlineID14-4
Temperature [K]100
Detector technologyCCD
Collection date2013-09-12
DetectorADSC QUANTUM 315
Wavelength(s)0.97935
Spacegroup nameC 1 2 1
Unit cell lengths222.000, 107.500, 165.800
Unit cell angles90.00, 114.00, 90.00
Refinement procedure
Resolution46.700 - 1.700
R-factor0.14724
Rwork0.146
R-free0.17571
Structure solution methodSAD
RMSD bond length0.024
RMSD bond angle2.289
Data reduction softwareXDS
Data scaling softwareXDS
Phasing softwareSHELXCD
Refinement softwareREFMAC (5.7.0032)
Data quality characteristics
 Overall
Low resolution limit [Å]46.700
High resolution limit [Å]1.700
Number of reflections389746
<I/σ(I)>16.1
Completeness [%]99.6
Redundancy3.1
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP2851:1 ratio of 0.14M Na/K-tartrate, 12% PEG 3350 with 9 mg/ml ZGAL_3152

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