5OPQ
A 3,6-anhydro-D-galactosidase produced by Zobellia galactanivorans. This is an exo-lytic enzyme that hydrolyzes terminal 3,6-anhydro-D-galactose from the non-reducing end of carrageenan oligosaccharides.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-09-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97935 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 222.000, 107.500, 165.800 |
Unit cell angles | 90.00, 114.00, 90.00 |
Refinement procedure
Resolution | 46.700 - 1.700 |
R-factor | 0.14724 |
Rwork | 0.146 |
R-free | 0.17571 |
Structure solution method | SAD |
RMSD bond length | 0.024 |
RMSD bond angle | 2.289 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHELXCD |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 46.700 |
High resolution limit [Å] | 1.700 |
Number of reflections | 389746 |
<I/σ(I)> | 16.1 |
Completeness [%] | 99.6 |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 285 | 1:1 ratio of 0.14M Na/K-tartrate, 12% PEG 3350 with 9 mg/ml ZGAL_3152 |