5OPJ

Beta-L-arabinofuranosidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9796
Spacegroup name	P 43 21 2
Unit cell lengths	96.210, 96.210, 187.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.540 - 2.050
R-factor	0.17022
Rwork	0.168
R-free	0.20709
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.546
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXCD
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.540	2.090
High resolution limit [Å]	2.050	2.050
Number of reflections	56151	2753
<I/σ(I)>	17.33	2.11
Completeness [%]	100.0	100
Redundancy	72.37	74.92
CC(1/2)	0.999	0.831

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	200 mM ammonium formate, 20% PEG 3350, 500 mM L-arabinose