5OPG
Structure of the Hantaan virus Gn glycoprotein ectodomain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-03 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 110.906, 110.906, 180.578 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.207 - 2.150 |
| R-factor | 0.2019 |
| Rwork | 0.201 |
| R-free | 0.22660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fxu |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.447 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 78.420 | 78.420 | 2.210 |
| High resolution limit [Å] | 2.150 | 9.620 | 2.150 |
| Rmerge | 0.195 | 0.042 | 2.253 |
| Rmeas | 0.198 | 0.042 | 2.285 |
| Rpim | 0.032 | 0.007 | 0.377 |
| Total number of observations | 1184141 | ||
| Number of reflections | 30998 | 419 | 2259 |
| <I/σ(I)> | 14.8 | ||
| Completeness [%] | 100.0 | 99.8 | 100 |
| Redundancy | 38.2 | 32.7 | 36.4 |
| CC(1/2) | 0.999 | 1.000 | 0.883 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 298 | 1.6 M ammonium sulphate, 0.1 M citrate pH 4 |
