5OPE

Robo1 Ig1-4 crystals form 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2007-09-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9395
Spacegroup name	C 2 2 21
Unit cell lengths	43.457, 99.134, 247.175
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.760 - 2.540
R-factor	0.215
Rwork	0.212
R-free	0.27100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5o5g
RMSD bond length	0.009
RMSD bond angle	1.150
Data reduction software	XDS (Oct 2015)
Data scaling software	Aimless (1.9.31)
Phasing software	PHASER (2.5.7)
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.700
High resolution limit [Å]	2.500	2.500
Rpim	0.095	0.580
Total number of observations	61677
Number of reflections	17779	1772
<I/σ(I)>	4.9	1.3
Completeness [%]	97.9	82.7
Redundancy	3.5	2.6
CC(1/2)	0.980	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	0.5 M ammonium phosphate, 100 mM Na Critrate