5OPE
Robo1 Ig1-4 crystals form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9395 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 43.457, 99.134, 247.175 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.760 - 2.540 |
| R-factor | 0.215 |
| Rwork | 0.212 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5o5g |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.150 |
| Data reduction software | XDS (Oct 2015) |
| Data scaling software | Aimless (1.9.31) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.700 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rpim | 0.095 | 0.580 |
| Total number of observations | 61677 | |
| Number of reflections | 17779 | 1772 |
| <I/σ(I)> | 4.9 | 1.3 |
| Completeness [%] | 97.9 | 82.7 |
| Redundancy | 3.5 | 2.6 |
| CC(1/2) | 0.980 | 0.530 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.5 M ammonium phosphate, 100 mM Na Critrate |
