5OP6

Factor Inhibiting HIF (FIH) in complex with zinc and GSK128863

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-18
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 41 21 2
Unit cell lengths	86.210, 86.210, 147.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.305 - 2.450
R-factor	0.2049
Rwork	0.204
R-free	0.22740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h2k
RMSD bond length	0.005
RMSD bond angle	0.569
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.480	2.580
High resolution limit [Å]	2.450	2.450
Rmerge	0.138	1.250
Rpim	0.042	0.390
Number of reflections	21126	3014
<I/σ(I)>	11.5	2
Completeness [%]	100.0	100
Redundancy	11.5	11.1
CC(1/2)	0.998	0.570

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	300	0.1M HEPES pH7.5, 1.1M ammonium sulfate, 2.5% PEG 400