5OP6
Factor Inhibiting HIF (FIH) in complex with zinc and GSK128863
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-18 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 86.210, 86.210, 147.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.305 - 2.450 |
R-factor | 0.2049 |
Rwork | 0.204 |
R-free | 0.22740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1h2k |
RMSD bond length | 0.005 |
RMSD bond angle | 0.569 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.480 | 2.580 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.138 | 1.250 |
Rpim | 0.042 | 0.390 |
Number of reflections | 21126 | 3014 |
<I/σ(I)> | 11.5 | 2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 11.5 | 11.1 |
CC(1/2) | 0.998 | 0.570 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 300 | 0.1M HEPES pH7.5, 1.1M ammonium sulfate, 2.5% PEG 400 |