5OP3
Designed Ankyrin Repeat Protein (DARPin) NDNH-1 selected by directed evolution against Lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96858 |
| Spacegroup name | H 3 |
| Unit cell lengths | 120.746, 120.746, 24.653 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.856 - 1.359 |
| R-factor | 0.1341 |
| Rwork | 0.133 |
| R-free | 0.16050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3oos |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.194 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 60.373 | 60.373 | 1.383 |
| High resolution limit [Å] | 1.359 | 3.690 | 1.359 |
| Rmerge | 0.077 | 0.041 | 1.392 |
| Rmeas | 0.080 | 0.042 | 1.486 |
| Rpim | 0.018 | 0.009 | 0.513 |
| Total number of observations | 489874 | ||
| Number of reflections | 28830 | ||
| <I/σ(I)> | 18.7 | ||
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 17 | 20 | 8.3 |
| CC(1/2) | 0.999 | 0.999 | 0.568 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 298 | 0.02 M CaCl2, 0.1 M Na Acet 4.6 pH, 30 %v/v MPD |
