5OP0

Structure of Prim-PolC from Mycobacterium smegmatis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-11
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97949
Spacegroup name	P 1
Unit cell lengths	51.550, 56.430, 64.450
Unit cell angles	97.17, 100.20, 90.64

Refinement procedure

Resolution	44.771 - 1.840
R-factor	0.187
Rwork	0.186
R-free	0.20920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2iry
RMSD bond length	0.005
RMSD bond angle	0.743
Data reduction software	xia2
Data scaling software	XSCALE
Phasing software	PHASER (2.6.1)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.771	44.770	1.890
High resolution limit [Å]	1.840	8.230	1.840
Rmerge	0.084	0.038	0.510
Rmeas	0.119	0.066	0.860
Number of reflections	110615	1292	8159
<I/σ(I)>	5.1	11.4	1.26
Completeness [%]	96.9	96.2	95.4
Redundancy	1.409	2.9	2.6
CC(1/2)	0.989	0.990	0.493

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	285	50mM Tris HCl, 200mM Ammonium Chloride, 10mM Calcium Chloride, 30% (w/v) PEG 4000