5OOZ
XFEL structure of the on state of a reversibly photoswitching fluorescent protein determined using the grease injection method
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SACLA BEAMLINE BL3 |
| Synchrotron site | SACLA |
| Beamline | BL3 |
| Temperature [K] | 293.15 |
| Detector technology | CCD |
| Collection date | 2016-05-13 |
| Detector | MPCCD |
| Wavelength(s) | 1.76 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.800, 74.600, 79.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.300 - 1.920 |
| R-factor | 0.15964 |
| Rwork | 0.156 |
| R-free | 0.22863 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.957 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 54.300 |
| High resolution limit [Å] | 1.911 |
| Number of reflections | 18901 |
| <I/σ(I)> | 13.77 |
| Completeness [%] | 99.7 |
| Redundancy | 17.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 293.15 | 0.2 M lithium sulfate 0.1 M Tris-Cl pH 8.5 23-30% PEG 3350 |
