5OOY
Designed Ankyrin Repeat Protein (DARPin) VHAH-1 in complex with Lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97626 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.773, 81.052, 89.387 |
| Unit cell angles | 90.00, 94.01, 90.00 |
Refinement procedure
| Resolution | 44.580 - 1.720 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5oov |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 81.052 | 81.052 | 1.747 |
| High resolution limit [Å] | 1.718 | 4.662 | 1.718 |
| Rmerge | 0.075 | 0.041 | 0.969 |
| Rmeas | 0.089 | 0.049 | 1.148 |
| Rpim | 0.048 | 0.027 | 0.611 |
| Total number of observations | 185561 | 8717 | 9624 |
| Number of reflections | 55206 | ||
| <I/σ(I)> | 8.8 | 21 | 1.3 |
| Completeness [%] | 98.9 | 97.4 | 98.6 |
| Redundancy | 3.4 | 3.1 | 3.4 |
| CC(1/2) | 0.997 | 0.997 | 0.556 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 298 | 10% PEG3000, 0.1M Imidazole pH=8.0, 0.2M LiSO4 |
