5OOV
Designed Ankyrin Repeat Protein (DARPin) ETVD-1 in complex with Lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96864 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.913, 61.661, 53.344 |
| Unit cell angles | 90.00, 105.63, 90.00 |
Refinement procedure
| Resolution | 52.065 - 1.365 |
| R-factor | 0.1499 |
| Rwork | 0.148 |
| R-free | 0.17940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5oos |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.849 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 52.065 | 52.065 | 1.389 |
| High resolution limit [Å] | 1.365 | 3.705 | 1.365 |
| Rmerge | 0.052 | 0.040 | 1.247 |
| Rmeas | 0.053 | 0.041 | 1.306 |
| Rpim | 0.012 | 0.010 | 0.385 |
| Total number of observations | 1140622 | 61142 | 36470 |
| Number of reflections | 66462 | ||
| <I/σ(I)> | 26.8 | 70.4 | 1.7 |
| Completeness [%] | 99.3 | 99.9 | 95.7 |
| Redundancy | 17.2 | 17.8 | 11.4 |
| CC(1/2) | 0.999 | 0.999 | 0.819 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 298 | 0.2M Na-Tartrate, 14.7% PEG3350 |
