5OOV
Designed Ankyrin Repeat Protein (DARPin) ETVD-1 in complex with Lysozyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.96864 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 100.913, 61.661, 53.344 |
Unit cell angles | 90.00, 105.63, 90.00 |
Refinement procedure
Resolution | 52.065 - 1.365 |
R-factor | 0.1499 |
Rwork | 0.148 |
R-free | 0.17940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5oos |
RMSD bond length | 0.007 |
RMSD bond angle | 0.849 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 52.065 | 52.065 | 1.389 |
High resolution limit [Å] | 1.365 | 3.705 | 1.365 |
Rmerge | 0.052 | 0.040 | 1.247 |
Rmeas | 0.053 | 0.041 | 1.306 |
Rpim | 0.012 | 0.010 | 0.385 |
Total number of observations | 1140622 | 61142 | 36470 |
Number of reflections | 66462 | ||
<I/σ(I)> | 26.8 | 70.4 | 1.7 |
Completeness [%] | 99.3 | 99.9 | 95.7 |
Redundancy | 17.2 | 17.8 | 11.4 |
CC(1/2) | 0.999 | 0.999 | 0.819 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 298 | 0.2M Na-Tartrate, 14.7% PEG3350 |