5OOU
Designed Ankyrin Repeat Protein (DARPin) YTRL-1 selected by directed evolution against Lysozyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 103.500, 103.500, 96.130 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.817 - 2.104 |
R-factor | 0.1864 |
Rwork | 0.185 |
R-free | 0.20630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5oos |
RMSD bond length | 0.002 |
RMSD bond angle | 0.484 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 65.557 | 65.557 | 2.140 |
High resolution limit [Å] | 2.104 | 5.710 | 2.104 |
Rmerge | 0.100 | 0.054 | 2.485 |
Rmeas | 0.103 | 0.056 | 2.551 |
Rpim | 0.024 | 0.014 | 0.569 |
Total number of observations | 343934 | 16385 | 17894 |
Number of reflections | 18216 | ||
<I/σ(I)> | 16.5 | 40.5 | 1.4 |
Completeness [%] | 100.0 | 100 | 98.9 |
Redundancy | 18.9 | 15.7 | 20 |
CC(1/2) | 0.999 | 0.999 | 0.475 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 298 | 1.5M AmSO4, 0.1M Tris pH=8.5, 12% glycerol |