5OOS
Designed Ankyrin Repeat Protein (DARPin) YTRL-2 selected by directed evolution against Lysozyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-01 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97949 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 102.487, 102.487, 96.714 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 65.390 - 2.260 |
R-factor | 0.198 |
Rwork | 0.196 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.080 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 65.393 | 65.393 | 2.267 |
High resolution limit [Å] | 2.259 | 10.472 | 2.259 |
Rmerge | 0.103 | 0.024 | 3.146 |
Rmeas | 0.104 | 0.024 | 3.176 |
Rpim | 0.014 | 0.004 | 0.431 |
Total number of observations | 764056 | ||
Number of reflections | 14609 | ||
<I/σ(I)> | 33.4 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 52.3 | 36.1 | 53.4 |
CC(1/2) | 1.000 | 1.000 | 0.674 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 298 | 30% PEG3000, 0.1M Tris pH=7, 0.2M NaCl |