5OOR
Structure of CHK1 10-pt. mutant complex with staurosporine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | BRUKER D8 QUEST |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2015-06-29 |
Detector | BRUKER PHOTON 100 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.814, 65.699, 54.034 |
Unit cell angles | 90.00, 101.64, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.900 |
R-factor | 0.1666 |
Rwork | 0.165 |
R-free | 0.20780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5oop |
RMSD bond length | 0.019 |
RMSD bond angle | 2.265 |
Data reduction software | SAINT |
Data scaling software | SADABS |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.770 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.108 | 0.474 |
Number of reflections | 28254 | 4133 |
<I/σ(I)> | 9.05 | 2.01 |
Completeness [%] | 98.9 | 97.7 |
Redundancy | 4 | 2.53 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 7% PEG 8000, 0.1 M MES buffer pH 6.5, 20% ethylene glycol |