5OOK
Structure of A. marina Phycocyanin contains overlapping isoforms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-30 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 63 |
| Unit cell lengths | 152.806, 152.806, 39.312 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 132.330 - 2.100 |
| R-factor | 0.19418 |
| Rwork | 0.193 |
| R-free | 0.21517 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cpc |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.236 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0171) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 132.330 | 2.175 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.069 | 0.555 |
| Rpim | 0.033 | 0.255 |
| Number of reflections | 31134 | 3105 |
| <I/σ(I)> | 10.6 | 2.7 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 9.7 | 10.5 |
| CC(1/2) | 0.999 | 0.920 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M HEPES pH 6.5, 0.1M MgCl2, 9% PEG 2K |
