5OOA
Streptomyces PAC13 (Y89F) with uridine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-02-17 |
Detector | RIGAKU SATURN A200 |
Wavelength(s) | 1.54178 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 66.792, 66.792, 54.560 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 57.840 - 1.600 |
R-factor | 0.15932 |
Rwork | 0.158 |
R-free | 0.18537 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.255 |
Data reduction software | XDS |
Data scaling software | Aimless |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.840 | 1.710 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 17880 | 2560 |
<I/σ(I)> | 37.3 | 7.6 |
Completeness [%] | 95.3 | 76.8 |
Redundancy | 3.3 | 1.7 |
CC(1/2) | 0.998 | 0.858 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.2 M ammonium fluoride, 20 % w/v PEG-3350 |