5OOA
Streptomyces PAC13 (Y89F) with uridine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-17 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 66.792, 66.792, 54.560 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 57.840 - 1.600 |
| R-factor | 0.15932 |
| Rwork | 0.158 |
| R-free | 0.18537 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.255 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.840 | 1.710 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 17880 | 2560 |
| <I/σ(I)> | 37.3 | 7.6 |
| Completeness [%] | 95.3 | 76.8 |
| Redundancy | 3.3 | 1.7 |
| CC(1/2) | 0.998 | 0.858 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.2 M ammonium fluoride, 20 % w/v PEG-3350 |
