5OO9
Streptomyces PAC13 (Y55F) with uridine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-22 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 66.604, 66.604, 54.257 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 57.680 - 1.590 |
| R-factor | 0.16195 |
| Rwork | 0.160 |
| R-free | 0.20003 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.223 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.680 | 1.680 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Number of reflections | 17643 | 2002 |
| <I/σ(I)> | 42.2 | 8.4 |
| Completeness [%] | 92.8 | 67.5 |
| Redundancy | 3.8 | 1.8 |
| CC(1/2) | 0.999 | 0.820 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1 M MES pH 6.5, 0.2 M NH4SO4, 30 % w/v 5K PEGMME |
