5OO5
Streptomyces PAC13 with uridine uronic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-17 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 66.489, 66.489, 55.232 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 57.580 - 1.780 |
| R-factor | 0.15508 |
| Rwork | 0.153 |
| R-free | 0.19512 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.222 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.850 | 1.930 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.050 | 0.248 |
| Number of reflections | 13723 | 2788 |
| <I/σ(I)> | 19.4 | 5 |
| Completeness [%] | 99.3 | 99.3 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.8 | 293 | 16% PEG3350,0.1M MgCl2,0.2MBis-Tris pH 8.8 |
