5OO2
Crystal structure of Mycolicibacterium hassiacum glucosylglycerate hydrolase (MhGgH) E419A variant in complex with glucosylglycolate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-09 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.98011 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 86.953, 157.676, 87.556 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.912 - 2.060 |
R-factor | 0.1642 |
Rwork | 0.162 |
R-free | 0.20210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ohz |
RMSD bond length | 0.010 |
RMSD bond angle | 1.030 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.060 | 2.100 |
High resolution limit [Å] | 2.060 | 2.060 |
Rmerge | 0.124 | 1.410 |
Rmeas | 0.135 | 1.526 |
Rpim | 0.052 | 0.579 |
Number of reflections | 75133 | |
<I/σ(I)> | 8.2 | 1.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.7 | 6.9 |
CC(1/2) | 0.997 | 0.575 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Tris-Bicine pH 8.5, amino acids, PEG 4000, glycerol |