5ONX
Resting state copper nitrite reductase determined by serial femtosecond rotation crystallography
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SACLA BEAMLINE BL3 |
| Synchrotron site | SACLA |
| Beamline | BL3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-23 |
| Detector | RAYONIX MX225-HS |
| Wavelength(s) | 1.242 |
| Spacegroup name | H 3 |
| Unit cell lengths | 89.970, 89.970, 143.560 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.840 - 1.600 |
| R-factor | 0.18747 |
| Rwork | 0.185 |
| R-free | 0.22596 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oe1 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.758 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.840 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 57138 | 5685 |
| <I/σ(I)> | 4.7 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 132.3 | 47 |
| CC(1/2) | 0.975 | 0.502 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 10% w/v PEG 550 MME, 10 mM ZnSO4, 100 mM MES buffer pH 6.5 |
