5ONU
Trimeric OmpU structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 146 |
Detector technology | PIXEL |
Collection date | 2015-04-26 |
Detector | DECTRIS PILATUS3 R CdTe 300K-W |
Wavelength(s) | 0.9173 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 129.880, 153.470, 81.010 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 62.731 - 2.220 |
R-factor | 0.2092 |
Rwork | 0.208 |
R-free | 0.23570 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 1.002 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 64.950 |
High resolution limit [Å] | 2.220 |
Number of reflections | 80557 |
<I/σ(I)> | 14.7 |
Completeness [%] | 99.8 |
Redundancy | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 289 | 0.1M lithium sulfate monohydrate, 0.1M sodium acetate trihydrate pH4.6, and 1M ammonium phosphate monobasic |