5ONM
Crystal Structure of Ectoine Synthase from P. lautus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-21 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.988 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 71.130, 71.130, 68.660 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.565 - 1.520 |
| R-factor | 0.1776 |
| Rwork | 0.176 |
| R-free | 0.20280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5bxx |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.405 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.565 |
| High resolution limit [Å] | 1.520 |
| Number of reflections | 31125 |
| <I/σ(I)> | 19.2 |
| Completeness [%] | 99.3 |
| Redundancy | 18.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 4.2 | 298 | 0.2 M ammonium sulfate, 0.1 M phosphate citrate pH 4.2, 20% (v/v) PEG 300, 10% (v/v) glycerol. |
