5ONK
Native YndL
Experimental procedure
| Experimental method | LAUE |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-10 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.6 - 2.3 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.670, 46.860, 100.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.200 - 1.030 |
| R-factor | 0.16726 |
| Rwork | 0.166 |
| R-free | 0.18721 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a9l |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.457 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 34.200 |
| High resolution limit [Å] | 1.030 |
| Number of reflections | 76651 |
| <I/σ(I)> | 18.67 |
| Completeness [%] | 85.0 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1 Ammonium sulphate, 0.15 Sodium citrate |
