5ONF
The ENTH domain from epsin-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-12-06 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.127 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 68.929, 119.363, 129.542 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.110 - 2.800 |
| R-factor | 0.27236 |
| Rwork | 0.272 |
| R-free | 0.28470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 27.000 |
| High resolution limit [Å] | 2.750 |
| Number of reflections | 13379 |
| <I/σ(I)> | 11.8 |
| Completeness [%] | 99.1 |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.2 M CaCl2, 25 % PEG 3350 and 0.1 M Tris pH 8.5. |
