5ON7

The ENTH domain from epsin-2 in complex with phosphatidylinositol 4,5-bisphosphate (PIP2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-22
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.03320
Spacegroup name	F 4 3 2
Unit cell lengths	211.357, 211.357, 211.357
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.709 - 3.350
R-factor	0.2868
Rwork	0.286
R-free	0.30220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.313
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	120.000	8.850
High resolution limit [Å]	3.350	3.350
Rmerge	0.134	1.620
Rpim	0.053	1.040
Number of reflections	7571
<I/σ(I)>	9.4	0.9
Completeness [%]	99.2	97.1
Redundancy	6.2
CC(1/2)		0.340

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	0.1 M MES, pH 6.5, 0.1 M NaCl, 1.45 M ammonium sulfate