5OMM

GII.10 Vietnam 026 protruding domain in complex with Nanobody Nano-14

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-23
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	C 1 2 1
Unit cell lengths	97.512, 94.209, 101.284
Unit cell angles	90.00, 112.70, 90.00

Refinement procedure

Resolution	48.497 - 1.700
R-factor	0.1662
Rwork	0.166
R-free	0.19290
RMSD bond length	0.008
RMSD bond angle	1.164
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.497	48.497	1.750
High resolution limit [Å]	1.650	4.920	1.650
Rmerge	0.064	0.027	0.561
Rmeas	0.072	0.030	0.628
Total number of observations	488528
Number of reflections	99684	3904	15617
<I/σ(I)>	16.55	49.28	2.78
Completeness [%]	98.0	99.2	95.3
Redundancy	4.901	4.904	4.887
CC(1/2)	0.999	0.999	0.806

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	0.1 M sodium citrate, 20% (w/v) PEG3000