5OMJ
R2-like ligand-binding oxidase with aerobically reconstituted metal cofactor after photoconversion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-30 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 55.625, 97.312, 127.984 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.656 - 2.005 |
| R-factor | 0.2054 |
| Rwork | 0.202 |
| R-free | 0.26370 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4hr0 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.187 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.130 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.137 | 2.325 |
| Number of reflections | 23693 | 3747 |
| <I/σ(I)> | 11.37 | 0.77 |
| Completeness [%] | 99.8 | 98.9 |
| Redundancy | 6.6 | 6.6 |
| CC(1/2) | 0.998 | 0.323 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 27.5% (w/v) PEG1500, 0.1 M HEPES-Na pH 7.5 |
