5OMC

Crystal structure of K. lactis Ddc2 N-terminus in complex with S. cerevisiae Rfa1 (K45E mutant) N-OB domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99999
Spacegroup name	P 21 21 21
Unit cell lengths	34.420, 94.590, 170.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.290 - 2.380
R-factor	0.2036
Rwork	0.201
R-free	0.26110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5omb
RMSD bond length	0.010
RMSD bond angle	1.130
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.11.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.450
High resolution limit [Å]	2.380	2.380
Number of reflections	21408	1199
<I/σ(I)>	5.87	0.99
Completeness [%]	92.1	69.9
Redundancy	3.77	2.92
CC(1/2)	0.993	0.355

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2 M lithium sulfate monohydrate, 0.1 M Bis-Tris, 25% PEG 3,350