5OMB

Crystal structure of K. lactis Ddc2 N-terminus in complex with S. cerevisiae Rfa1 N-OB domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-15
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	34.050, 94.940, 170.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.760 - 1.940
R-factor	0.1871
Rwork	0.185
R-free	0.22040
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.030
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AutoSol
Refinement software	BUSTER (2.11.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.990
High resolution limit [Å]	1.940	1.940
Number of reflections	41015	2799
<I/σ(I)>	12.1	1.6
Completeness [%]	98.0	91.3
Redundancy	11.4	11
CC(1/2)	0.999	0.605

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.2 M ammonium citrate tribasic, 20% PEG 3350