5OLX
5-fluorotryptophan labeled beta-phosphoglucomutase in a closed conformation, orthorhomic crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-09-01 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.969 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.530, 54.164, 104.225 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.380 |
R-factor | 0.15665 |
Rwork | 0.154 |
R-free | 0.20936 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.028 |
RMSD bond angle | 2.472 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.000 | 1.460 |
High resolution limit [Å] | 1.380 | 1.380 |
Rmerge | 0.145 | 0.700 |
Rpim | 0.071 | 0.340 |
Number of reflections | 43356 | 5975 |
<I/σ(I)> | 6.7 | 1.9 |
Completeness [%] | 98.0 | 94.4 |
Redundancy | 4.8 | 4.7 |
CC(1/2) | 0.993 | 0.523 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 25% PEG4000, 100 mM Na acetate |