5OLP
Galacturonidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9163 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 127.545, 127.545, 263.641 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.730 - 2.000 |
| R-factor | 0.18341 |
| Rwork | 0.182 |
| R-free | 0.21534 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.510 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.730 | 2.040 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 73408 | 4477 |
| <I/σ(I)> | 12.8 | 1.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 26.8 | 28 |
| CC(1/2) | 0.998 | 0.620 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200 mM sodium chloride, 100 mM Bis-Tris buffer pH 5.5 and 25% PEG 3350 |
