5OLC
Crystal structure of the 3,6-anhydro-D-galactonate cycloisomerase from Zobellia galactanivorans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-30 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.98011 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 85.840, 154.070, 150.870 |
Unit cell angles | 90.00, 104.38, 90.00 |
Refinement procedure
Resolution | 49.170 - 2.790 |
R-factor | 0.233 |
Rwork | 0.231 |
R-free | 0.26600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hpn |
RMSD bond length | 0.010 |
RMSD bond angle | 1.190 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.170 | 2.870 |
High resolution limit [Å] | 2.790 | 2.790 |
Number of reflections | 93118 | |
<I/σ(I)> | 3.97 | 0.87 |
Completeness [%] | 99.0 | 95.6 |
Redundancy | 3.6 | 3.6 |
CC(1/2) | 0.978 | 0.473 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | Optimized conditions were a 1:1 ratio of 0.2 M tri potassium citrate, 20% PEG 3350 to 25 mg/ml ZGAL_3156. |