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5OKR

Conservatively refined structure of Gan1D-WT, a putative 6-phospho-beta-galactosidase from Geobacillus stearothermophilus, in complex with 6-phospho-beta-glucose

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU FR-X
Temperature [K]100
Detector technologyIMAGE PLATE
Collection date2014-01-01
DetectorRIGAKU RAXIS HTC
Wavelength(s)1.54
Spacegroup nameC 1 2 1
Unit cell lengths107.260, 68.950, 153.010
Unit cell angles90.00, 100.38, 90.00
Refinement procedure
Resolution27.880 - 2.150
R-factor0.1686
Rwork0.166
R-free0.21530
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)5okh
RMSD bond length0.007
RMSD bond angle0.841
Data reduction softwareiMOSFLM
Data scaling softwareAimless
Phasing softwarePHASER
Refinement softwarePHENIX ((1.10.1_2155: ???))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]27.8802.210
High resolution limit [Å]2.1502.150
Rmerge0.1100.454
Number of reflections5884112183
<I/σ(I)>6.92
Completeness [%]98.191.3
Redundancy3.92.9
CC(1/2)0.9910.839
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6.529316-19% PEG 8K, 3% MPD, 0.1M imidazole buffer pH 6.5

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