5OKC

Crystal structure of the Ctf18-1-8 module from Ctf18-RFC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-30
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	58.515, 164.184, 59.613
Unit cell angles	90.00, 90.50, 90.00

Refinement procedure

Resolution	48.235 - 2.300
R-factor	0.2031
Rwork	0.200
R-free	0.25970
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.629
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	CRANK2
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.240	48.240	2.380
High resolution limit [Å]	2.300	9.200	2.300
Rmerge	0.243	0.110	2.366
Total number of observations	337988
Number of reflections	49802	802	4570
<I/σ(I)>	6.9
Completeness [%]	99.9	99.3	100
Redundancy	6.8	6.3	6.9
CC(1/2)	0.992	0.991	0.481

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.8	293	16% PEG 3350, 0.1 M HEPES pH 7.0, 0.2 M potassium nitrate