5OK0
Structure of the D10N mutant of beta-phosphoglucomutase from Lactococcus lactis trapped with native reaction intermediate beta-glucose 1,6-bisphosphate to 2.2A resolution.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-10 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97950 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.810, 54.940, 103.340 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.510 - 2.150 |
R-factor | 0.2235 |
Rwork | 0.220 |
R-free | 0.29234 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wf5 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.495 |
Data reduction software | XDS |
Data scaling software | xia2 |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.510 | 2.190 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.339 | 1.683 |
Rpim | 0.148 | 0.721 |
Number of reflections | 11990 | 598 |
<I/σ(I)> | 5.3 | 1.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.2 | 6.4 |
CC(1/2) | 0.980 | 0.391 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 291 | 24-34% PEG 4000 200mM Tris pH 7.5 200mM sodium acetate harvested after 1 week |