5OJI
Crystal structure of the dehydrogenase/reductase SDR family member 4 (DHRS4) from Caenorhabditis elegans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-05-21 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.976261 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 94.750, 94.750, 132.560 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 66.280 - 1.600 |
R-factor | 0.2031 |
Rwork | 0.202 |
R-free | 0.22940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3o4r |
RMSD bond length | 0.006 |
RMSD bond angle | 1.206 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.280 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.181 | 0.963 |
Rmeas | 0.185 | 0.998 |
Number of reflections | 79504 | |
<I/σ(I)> | 16.8 | |
Completeness [%] | 99.3 | 89.2 |
Redundancy | 25 | 14 |
CC(1/2) | 0.999 | 0.272 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 10% (w/v) PEG 8000 200 mM NaCl 100 mM K/Na-phosphate, pH 6.2 |