5OJF

Crystal Structure of KLC2-TPR domain (fragment [A1-B6]

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.980097
Spacegroup name	C 1 2 1
Unit cell lengths	97.620, 116.470, 108.110
Unit cell angles	90.00, 99.51, 90.00

Refinement procedure

Resolution	20.000 - 3.400
R-factor	0.234
Rwork	0.232
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ceq 3nf1
RMSD bond length	0.010
RMSD bond angle	1.190
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.140	3.590
High resolution limit [Å]	3.400	3.400
Rmeas	0.236	1.168
Number of reflections	16593
<I/σ(I)>	4.55	1.08
Completeness [%]	99.2	95.7
Redundancy	3.6	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	12 % PEG 3350, 0.5 M Phos. pH 7.5 Glycerol 25 %