5OHU
The X-ray Structure of Lytic Transglycosylase Slt from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-12 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 63 |
| Unit cell lengths | 163.981, 163.981, 56.706 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.337 - 2.200 |
| R-factor | 0.1843 |
| Rwork | 0.182 |
| R-free | 0.23780 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.877 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.340 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rpim | 0.050 | 0.860 |
| Number of reflections | 44540 | |
| <I/σ(I)> | 14.8 | 1.35 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19.4 | 19.3 |
| CC(1/2) | 0.990 | 0.720 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | K/Na phosphate 1M |
