5OHO

Crystal structure of the KOWx-KOW4 domain of human DSIF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00003056744
Spacegroup name	P 21 21 21
Unit cell lengths	50.286, 54.403, 73.065
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.635 - 1.601
R-factor	0.1767
Rwork	0.174
R-free	0.22130
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.862
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.635	43.635	1.700
High resolution limit [Å]	1.600	4.780	1.600
Rmerge	0.047	0.027	1.336
Rmeas	0.051	0.029	1.452
Number of reflections	50856	1915	8120
<I/σ(I)>	15.88	53.72	0.94
Completeness [%]	99.7	99.8	98.7
Redundancy	6.76	6.81	6.466
CC(1/2)	0.999	0.999	0.485

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1 M sodium cacodylate pH 6.5, 1.0 M tri-sodium citrate dihydrate